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KMID : 0363819910250020252
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Korean Journal of Nuclear Medicine
1991 Volume.25 No. 2 p.252 ~ p.258
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^(99m)Tc-HMPAO Labelled WBC Scan in Experimental Abscess by Labelling Autologous
Leukocytes with In-House-Synthesized HMPAO
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Abstract
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With HMPAO we have synthesized in our laboratory, we labelled 99mTc to canine leukocytes. Experimental abscess made by subcutaneous injection with Staphylococcus aureus was imaged with these "Tc labelled leukocytes. Labelling efficiency of HMPAO with "mTc was 66.2%±14.6% (N = 9). Labelling efficiency of leukocytes with "mTc-HMPAO was 54% ± 7.7% (N = 7). Cell bound radioactivity in 99mTc-HMPAO labelled leukocytes was around 80% when these cells were incubated in plasma in vitro at 37°C for 5 hours. In vivo cell bound activity was over 80% at 24 hours after injection. One day and four days after inoculation, uptake at the inflammatory focus was found with "Tc labelled leukocytes. Uptake showed up in 4 hour image, and the uptake at the lesion was most prominent in 24 hour image. These findings show that in-house-synthesized HMPAO could be used for labelling leukocytes with 99mTc, and that 99mTc-HAIPAO-labelled leukocytes were so stable and viable that inflammatory focus could be visualized with these 99mTc-labelled leukocytes.
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KEYWORD
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